Human beings are inevitably exposed to toxic substances as a result of influences of potential contamination factors in the environment, food and medicines, which poses a threat to human health. In order to effectively screen and prevent the exposure or intake of such substances, it is necessary to develop in vitro assays for the detection and toxicity evaluation of toxic substances. High content screening (HCS) has been recognized as an important tool for toxicity testing and risk assessment of compounds due to its high throughput and automation advantages, and has been widely used in in vitro toxicology research. In this review, we described the system components of HCS and its workflow in toxicity screening and toxicity evaluation by focusing on cases of their application in toxicity detection and evaluation studies, including the cytotoxicity, hepatotoxicity, nephrotoxicity, genotoxicity, neurotoxicity, cardiotoxicity, and developmental toxicity. In addition, the applications and developments of 
machine learning in HCS were explored, especially to the advantages of supervised and unsupervised machine learning strategies for high throughput image screening and data analysis. Finally, the future applications of HCS in toxicity screening and evaluation are outlined, especially in terms of binding new models and gene editing technology.

Metabolism-related diseases are chronic diseases caused by genetic and environmental factors. The symptoms include insulin resistance, abnormal blood glucose and lipid levels, and elevated blood pressure. This type of illness has become a major threat to human health, and there is an urgent need to find effective treatments. Advanced glycation end products (AGE) are a group of complex and heterogeneous compounds that result from reduced interactions between the carbonyl groups of  sugar and the free amino groups of proteins, lipids, and nucleic acids. Increasing evidence shows that AGE and its receptor (RAGE) are involved in the occurrence and development of such metabolism-related diseases as hypertension, diabetes, and atherosclerosis. AGE can have adverse effects on tissues through non-receptor and receptor-mediated mechanisms. In the receptor-mediated mechanism, AGE interacts with RAGE to increase the production of oxygen free radicals and activate NF-κB so that more pro-inflammatory cells are expressed and released, leading to cell damage. This article reviews the research progress in interventions with AGE and RAGE in the treatment of hypertension, diabetes, and atherosclerosis from a metabolic perspective in the hope of exploring the potential of AGE and RAGE as therapeutic targets for metabolism-related diseases.

OBJECTIVE  To optimize hematopoietic stem cell transplantation therapy and provide support for drug research by investigating the dynamic process of hematopoietic and immune system reconstitution after bone marrow transplantation (BMT) in mice. METHODS  CD45.2+ C57BL/6 mice were used as recipient mice and randomly divided into the normal control group and transplantation group, with 30 mice in each. The transplantation group was irradiated by a lethal dose of cobalt-60 rays. Bone marrow cells were prepared from CD45.1⁺ C57BL/6 mice and transfused into recipient mice through the tail vein. Peripheral blood, spleens, lymph nodes, thymuses and bone marrow were collected at 1, 2, 4, 8 and 16 weeks after transplantation. Blood routine examination was performed with peripheral blood and total cell numbers in suspensions of other organs were counted by an automated cell counter. Cell classification analysis of white blood cells in peripheral blood, cell suspensions of other organs was performed by flow cytometry. RESULTS  Four weeks after BMT, the numbers of white blood cells and red blood cells in peripheral blood of recipient mice returned to the same level of or higher level than normal control (P<0.05). Although the number of platelets recovered significantly, it was still markedly lower than that of normal control until 16 weeks post BMT (P<0.05). In addition, the percentages of myeloid leukocytes and B cells in peripheral blood, spleens, lymph nodes, and bone marrow, as well as megakaryocytes and erythrocyte progenitor cells in bone marrow also returned to normal, and the majority of myeloid leukocytes and B cells were CD45.1⁺ cells from the donors. Eight weeks after BMT, T cells in peripheral blood, spleens, lymph nodes, thymuses, and bone marrow of recipient mice returned to normal, and CD45.1⁺ T cells were dominating. CONCLUSION  The hematopoietic and immune reconstitution of recipient mice is nearly completed eight weeks after BMT. However, the reconstruction speed of different kinds of cells and the reconstruction status of same kind of cell in different organs vary widely.

OBJECTIVE  To explore the applicablity of ′BlueScreen HC′(BSHC), a high throughput genotoxicity screening system based on human growth arrest and DNA damage inducible 45α(GADD45α) gene, in detecting the genotoxicity of migrants mixtures from food contact materials (FCM). METHODS  The 2000 bp sequence upstream of the open reading frame of human GADD45α gene was used as the promoter to construct the lentiviral plasmid pEZX-LvPG04, which was double labeled by purinamycin and Gausluciferase (Gluc), and the lentiviral plasmid was infected with human lymphoblastocyte TK6 to obtain a stable transmutation cell line TK6-Gluc.  Methyl methylate (MMS) at concentrations of 0, 1.56, 3.13, 6.25, 12.5, 25.0 and 50.0 mg·L^-1 was selected as the genotoxin without liver S9, cyclophosphamide (CTX) 0, 0.78, 1.56, 3.13, 6.25, 12.5, 25.0 mg·L^-1 was selected as the pre-genotoxin with liver S9, and dimethyl sulfoxide (DMSO) 0, 0.35, 0.69, 1.38, 2.75, 5.5 and 11.0 g·L^-1 was selected as the non-genotoxin. The constructed BSHC was verified with the above known genetic positive and negative substance respectively. Polybutyleneadipate-co-terephthalate (MS/PBAT) was tested using 4% (V/V) acetic acid, and 10%, 20%, 50% and 95% (V/V) ethanol as food simulants at 40 ℃ for 24 hours to obtain 5 multi-component migrants of MS/PBAT that were obtained by using DMSO as a solvent. TK6-Gluc cells were treated with 5 multi-component migrants of MS/PBAT at concentrations of 0, 0.38, 0.76, 1.53, 3.05, 6.10 and 12.20 g·L^-1 with or without liver S9. Cells were treated without liver S9 for 48 h. Cells treated with liver S9-mix were incubated for 3 h at a final concentration of 1% (V/V) liver S9 before being washed and re-suspended in fresh recovery media for another 45 h. After exposure, the cell viability was detected using the CCK-8 cell activity kit, and the Gluc Luminescence in the medium was detected with Secrete-PairTM Gaussia Luciferase Assay Kit. In addition, the mutagenicity on Salmonella typhimurium TA98 and TA100 was detected by micro-fluctuation Ames test with 5 multi-component migrants of MS/PBAT at concentrations of 3.05 and 12.20 g·L^-1. The in vitro mammalian cell chromosome aberration test was performed on CHL cells with 5 multi-component migrants of MS/PBAT at concentrations of 3.05 and 12.20 g·L^-1 to detect the chromosomal aberration. The results of genotoxicity were compared with those of BSHC. RESULTS  The lowest effect centration (LEC; <80% relative cell viability) and the coytotoxicity (<30% relative cell viability) was defined. A positive genotoxicity result threshold was determined at 1.8-fold relative induction. For the liver S9 protocol, the same process was followed, and the decision threshold derived was 1.5-fold relative Gluc induction. It is considered as genetic substance only when a positive genotoxicity result was reached and there was no cytotoxicity. Compared with the vehicle control group, no genotoxicity was observed at all concentration of DMSO by BSHC. MMS 12.5, 25.0 and 50.0 mg·L^-1 produced genotoxicity without liver S9 while CTX 6.25, 12.5 and 25.0 mg·L^-1 produced genotoxicity with liver S9. Significant cell growth inhibition was observed in 95% ethanol migrants of MS/PBAT at concentrations of 6.10 and 12.20 g·L^-1, and in 50% ethanol migrants of MS/PBAT at a concentration of 12.20 g·L^-1 without liver S9. No cytotoxicity with a relative cell viability below 30% was observed in any of the treatment groups, and no high expression of Gluc was observed. Therefore, none of the 5 multi-component migrants produced genotoxicity without liver S9. Significant cell growth inhibition was observed in 95% ethanol migrants of MS/PBAT at a concentration of 12.20 g·L^-1, and in 4% acetic acid migrants of MS/PBAT at concentrations of 6.10 and 12.20 g·L^-1 with liver S9. No cytotoxicity with a relative cell viability below 30% was observed in any of the treatment groups. No high expression of Gluc was observed. Therefore, none of the 5 multi-component migrants produced genotoxicity with liver S9. In the micro fluctuation Ames test, when 5 multi-component migrants of MS/PBAT were treated with concentrations of 3.05 and 12.20 g·L^-1 on TA98 and TA100 strains, there was no significant difference in the number of mutagenic positive wells compared with DMSO control group with or without liver S9,  indicating that no mutagenic effect was produced.  When CHL cells were treated with 5 multi-component migrants of MS/PBAT at concentration of 3.05 and 12.20 g·L^-1, compared with DMSO control group, there was no significant difference in chromosome aberration rate of CHL cells with or without liver S9. CONCLUSION  BSHC based on GADD45α gene has been established, which can be used for in vitro genotoxicity evaluation of migrants mixtures of FCM, but further exploration of its minimum effective concentrations is still needed, and more types of mixtures need to be applied for further validation.

The Hippo/YAP signaling pathway is an evolutionarily conserved protein kinase cascade that plays an important role in a variety of biological processes, such as cell proliferation and differentiation, organ growth and tissue regeneration. Fibrosis is a continuous and highly dynamic process characterized by excessive deposition of extracellular matrix, resulting in irreversible pathological changes that eventually lead to the failure of multiple tissues and organs. Targeted therapeutic strategies to ameliorate or reverse fibrosis are lacking. Studies have shown that the aberrantly activated Hippo/YAP signaling pathway may play a role in the development of fibrosis by regulating collagen deposition, fibroblast overproliferation, and epithelial cell differentiation, but the specific mechanism of action has not been fully elucidated. Targeting the Hippo/YAP signaling pathway involves two mechanisms: one is to target the upstream molecules of the Hippo/YAP signaling pathway, which is mainly achieved by inhibiting the activity of the core kinase or blocking the interaction with other molecules; the other is to target the downstream activities of YAP/TAZ and YAP/TAZ-TEAD in the Hippo/YAP signaling pathway. Studies have shown that the phosphorylation and subcellular localization of YAP/TAZ are significantly altered when tissue and organ damage occurs. This article is intended to review the current research on Hippo/YAP signaling pathway and its mediation of fibrosis in the lung, heart, liver, kidney, pancreas and skin in hopes of providing new ideas for studies on the pathogenesis of fibrosis and targeted therapeutic drugs.

Along with more research into the mechanisms of pulmonary fibrosis, anti-pulmonary fibrosis drugs under development are becoming diversified. Over the past decade, multiple phase Ⅱ/Ⅲ clinical trials have been terminated due to insufficient efficacy, with seven single-target monoclonal antibody drugs failing to meet expectations. In contrast, multi-target drugs such as nintedanib and pirfenidone have been successfully marketed, demonstrating favorable clinical outcomes. However, drugs 
directly targeting transforming growth factor-β have raised safety concerns. Ongoing phase Ⅲ candidates, such as lysophosphatidic acid receptor 1 antagonists and phosphodiesterase 4B inhibitors, do not directly intervene in the transforming growth factor-β signaling pathway. Given the potential limitations of single-target drugs, future drug development is expected to prioritize multi-target and multi-cell strategies while exploring synergistic multi-drug therapies. This article reviews the current clinical trials for anti-pulmonary fibrosis drugs worldwide and the challenges they face in order to provide references for the research and development of new anti-pulmonary fibrosis drugs.

Integrated Approaches to Testing and Assessment (IATA) is a toxicological assessment decision-making method that integrates existing information from various sources, including physical and chemical properties, animal testing, and non-animal testing. Through the evaluation and analysis of a series of iterative strategies, it ultimately obtains risk assessment conclusions, thereby providing a basis for risk management decisions regarding chemical substances. The use of IATA is becoming increasingly prevalent in such areas as ocular irritation and genotoxicity. This paper introduces the conceptual connotation of IATA, sorts out the framework elements and sequential processes, explains the commonly used framework construction methods, shares cases of application in various exposure scenarios, and finally envisions future research directions in order to provide better methodological support for the risk assessment of chemical substances.

With the emergence of high-throughput technology and massive toxicology data, toxicology research has entered the era of big data. How to efficiently integrate existingtoxicological data, clarify the toxic effects of chemicals, and use these patterns to providenew information, in order to achieve efficient prediction of the toxicity of new chemicalsubstances, is one of the cutting-edge issues in toxicology. In view of the high cost, low throughput and difficulty in revealing the mechanism information of traditional chemical toxicity testing methods, high throughput prediction models are urgently needed. Machine learning methods have been applied to toxicity testing, such as supervised learning models, unsupervised learning models, deep learning models, reinforcement learning models, and transfer learning models. Chemical characteristic data commonly used in machine learning models include chemical structure data, text data, toxicological genome data and image data. There is huge potential for applying machine learning to toxicity testing and machine learning methods have made some progress. However, current research focuses on the processing of data and development of  models, which has failed to produce a widely used and accepted method. In addition, the prediction accuracy of machine learning models is not only dependent on algorithms, but also affected by data quality, and the mutual promotion and development of algorithms and data quality remains a big challenge. In short, data 
processing and model construction in the field of toxicology require interdisciplinary cooperation and technological innovation. With the increasing perfection of toxicology databases and the continuous optimization of various model algorithms, the toxicity prediction of new chemicals based on machine learning models will become increasingly efficient and accurate, playing an important role in ensuring human health and environmental safety.

With the rapid development of industry and economy, the emergence of a large number of chemicals has made of risk management more difficult. Traditional risk assessment relies on animal experiments for toxicity testing. However, animal experiments are time-consuming, costly, and unable to meet the practical needs of risk assessment. The increasing maturity of toxicity testing alternative technologies signifies the possibility of rapid, sensitive, and accurate identification of chemical toxicity. This article focuses on the research and applications of alternative toxicity testing by reviewing the background, developments, and current research at home and abroad. It also discusses the progress in alternative testing methods in such areas as cosmetics and food safety risk assessment and explores the problems with the development of alternative testing technologies and risk assessment in China. This review aims to provide a reference for the system construction of cosmetics health risk assessment in China.

Antibody-based therapies are one of the crucial tumor-targeted therapies, enabling precise elimination of tumor cells by specifically binding to antigens on the tumor cell surface. However, their wide applications in solid tumor therapy are often limited by on-target toxicity. Recent advancements in antibody engineering have led to the development of novel tumor-targeted masking antibodies, which are specifically designed to address these limitations. Masking antibodies typically consist of an antibody domain, a masking domain and a linker. These antibodies are characterized by selective activation and other functional properties. Currently, various masking antibody technologies with distinct characteristics have been developed and have demonstrated favorable safety profiles in animal studies. This review summarizes the structure and characteristics of tumor-targeted masking antibodies outlines common masking technologies and their drug development in order to offer new lines of thought  for the design and development of next-generation tumor-targeted therapeutics.

Animal models are powerful tools for studying the mechanism of depressive disorders and screening antidepressants, but so far there is no model which can stimulate the clinical status of patients ideally. Here, we briefly introduced the research advances in classic animal models of depressive disorders, and focused on stress-related animal models, especially those induced by physical and social psychological stressors. The tests for evaluating animal depression behavior were reviewed. In this article, the strengths and weaknesses of each model were analyzed, and the precautions in its application were recommended. Finally, given the high heterogeneity of depressive disorders, this article elaborated on the research progress in models for subtypes of depressive disorders, such as treatment resistant depression, bipolar disorder, peripartum depression, and premenstrual syndrome.

OBJECTIVE  To investigate the mechanism by which salvianolic acid A (Sal A) reduces the inflammatory response and oxidative stress of BV2 cells injured by oxygen and glucose deprivation/reperfusion (OGD/R). METHODS  An OGD/R injury model of BV2 cells was established with sugar free Earle solution containing Na₂S₂O₄ 10 mmol·L^-1. Na₂S₂O₄ sugar free Earle solution was added and cultured in an incubator (37 ℃, 5%CO₂) for 1.5 h (oxygen glucose deprivation) before a normal medium was used for 24 h (reperfusion). Then, the cells were divided into the cell control group, OGD/R group, OGD/R+Sal A 1, 5 and 10 μmol·L^-1 group, OGD/R+ML385 group, OGD/R+ML385+Sal A 1, 5 and 10 μmol·L^-1 group and OGD/R+edaravone (Eda, 50 μmol·L^-1) group. After twenty-four hours of culture, the cell survival rate was measured by CCK8 kit. The contents of Interleukin-1β (IL-1β), IL-6, tumor necrosis factor-α (TNF-α), IL-10, IL-4 and transforming growth factor-β (TGF-β) in the cell supernatant were detected by ELISA. Reactive oxygen species (ROS) in cells was detected using the chemical fluorescence method. The contents of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and chloramphenicol acetyltransferase (CAT) in cells were determined with the colorimetric method. Protein expressions of Kelch like ECH-associated protein 1 (Keap1), nuclear factor erythroid-2 related factor 2 (Nrf2),Heme oxygenase-1 (HO-1), NADPH: quinone oxidoreductase-1 (NQO1) and p-nuclear factor kappa-B p65 (p-NF-κB p65) were detected by Western blotting. RESULTS  ① Compared with the cell control group, the cell survival rate of the OGD/R group was significantly decreased (P<0.01). Compared with the OGD/R group, the survival rates of OGD/R+Sal A 1, 5 and 10 μmol·L-1 groups were significantly increased (P<0.05, P<0.01). ② Compared with the cell control group, the contents of IL-1β, IL-6 and TNF-α were significantly increased, the contents of IL-10, IL-4 and TGF-β were significantly decreased, the contents of ROS and MDA were significantly increased, and the activities of SOD, CAT and GSH-Px were significantly decreased in the OGD/R group (P<0.01). Compared with the OGD/R group, the content of IL-6 was significantly decreased, the contents of IL-10, IL-4 and TGF-β were significantly increased, the contents of ROS and MDA were significantly decreased, and the activities of SOD, CAT and GSH-Px were significantly increased in OGD/R+Sal A 1, 5 and 10 μmol·L-1 and OGD/R+Eda groups (P<0.05, P<0.01). ③ Compared with the cell control group, the protein expression of p-NF-κB P65 in the OGD/R group was significantly increased (P<0.01). Compared with the OGD/R group, the protein expressions of Keap1 and cytoplasmic Nrf2 were significantly decreased, the expressions of nuclear Nrf2, HO-1 and NQO1 proteins were significantly increased, and the expression of p-NF-κB p65 protein was significantly decreased in OGD/R+Sal A 5 and 10 μmol·L^-1 and OGD/R+Eda groups (P<0.05, P<0.01). In OGD/R+ML385, OGD/R+ML385+Sal A 1, 5 and 10 μmol·L^-1 groups, the protein expression of Keap1 was significantly increased, the protein expressions of cytoplasmic Nrf2, nuclear Nrf2, HO-1 and NQO1 protein were significantly decreased, and the protein expression of p-NF-κB P65 was significantly increased (P<0.01). CONCLUSION  Sal A reduces the inflammatory response and oxidative stress of OGD/R injured BV2 cells possibly by activating the Keap1/Nrf2 pathway and inhibiting the NF-κB pathway.

Nanomaterials have been used in a variety of industries recently, involving foods, chemicals and biomedicine. There are multiple routes through which humans are exposed to nanomaterials, and their toxic effects deserve more attention. In vivo studies have confirmed that nanomaterials exposure can lead to toxicity in such target organs as the heart, liver, kidney, skin and nerve. The toxicity mechanism is related to changes in organelles such as the endoplasmic reticulum, lysosomes and mitochondria. Increasing studies suggest that mitochondria are critical targets for the toxicity of nanomaterials. Mitochondrial biogenesis serves as an important mechanism for maintaining mitochondrial homeostasis, which plays a vital role in the process of nanomaterials-induced cellular toxicity. This article summarizes the current research on the effects of nanomaterials on mitochondrial biogenesis, and elaborates the mechanism through which nanomaterials disrupt mitochondrial biogenesis by triggering oxidative stress, upsetting the homeostasis of calcium ion and disturbing toxicity pathways. This article is expected to provide a reference for toxicity testing and risk assessment of nanomaterials.

Food toxicology plays a crucial role in supporting scientific and technical aspects of food safety risk assessment. However, traditional methods relying on animal testing are becoming increasingly inadequate for identifying and evaluating emerging foods and unknown risks. There is a strong push worldwide towards the development of new approach methodologies (NAMs) based on non-animal testing methods. Policies and regulations related to NAMs are being standardized gradually in the European Union, the United States, and China. Some progress has also been made in applying these methodologies in food toxicology research in China. For instance, within the "Food Toxicology Program" at the National Center for Food Safety Risk Assessment, high-content and high-throughput in vitro hazard identification models employing model organisms like human macrophages, hepatocytes, adipocytes, embryonic stem cells, and zebrafish, as well as Toxicological Thresholds of Concern and quantitative in vitro to in vivo extrapolation based on physiologically-based toxicokinetic models have been established and applied. Nonetheless, new toxicological hazard identification technologies still face challenges such as inadequate elucidation of toxic mechanisms, insufficient collaborative research efforts, and inefficient translation of these findings into practical applications.

Special populations such as the elderly, pregnant women, children, and patients with impaired liver and kidney function have different physiological characteristics and drug processes from other patients. As a result during medication, these populations are vulnerable to drug-drug interactions in case of combined medications, which makes it more difficult of optimize drug treatment and develop new drugs. The physiologically based pharmacokinetic (PBPK) model can predict drug-drug interaction in special populations by altering metabolism-related physiological parameters such as metabolic 
enzymes, transporter activity and clearance. Gene polymorphisms, intestinal metabolism and other 
factors also affect the accuracy of model results. This review aims to to optimize clinical regimens for special populations and provide references for new drug development.

As antibiotic resistance becomes increasingly concerning, antimicrobial peptides, as a new type of antibiotic alternative, have attracted more attention. However, the low enzymatic stability of antimicrobial peptides severely limits their clinical applications. To address this issue, researchers have developed various structural modification strategies, including the introduction of unnatural amino acids, peptide chain cyclization and chemical group modification. This article reviews the basic principles and cases of the above modification strategies analyzes the advantages and disadvantages of different modification strategies and recommends ways these strategies can be optimized. In addition, this article predicts the developments of and potential challenges to strategies for enhancing enzymatic stability of antimicrobial peptides in the hope of providing references for subsequent research and development of antimicrobial peptides.

OBJECTIVE  To investigate the role of Bai Ling Long Zao An Shen formula (BLLZ) in sleep improvement in an environmental stress-induced insomnia rat model and explore its underlying mechanisms. METHODS  (1) Component analysis: the chemical constituents of the BLLZ extract were analyzed using ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS). (2) Evaluation of the sedative and hypnotic effect: ① Mice: 50 ICR mice were randomly divided into normal control group, BLLZ-L group (5, 10 and 20 g·kg^-1) and diazepam group (DZP, 3 mg·kg^-1). After five days of intragastric administration, pentobarbital sodium-induced righting reflex and locomotor activity tests were performed. ② Rats: 8 SD rats were implanted with electrodes and allowed to recover for seven days before baseline EEG data was collected over 24 h. A crossover design (7 d washout period) was employed,  with rats randomly assigned to the DZP (3 mg·kg^-1) and BLLZ (20 g·kg^-1) group. After five days of treatment, 24 h EEG recordings were obtained. (3) Insomnia model and interventions: ① 8 SD rats were allowed to recover for seven days post-surgery, followed by 6 h (14:00-20:00) baseline EEG recording. A 3×3 crossover design was used to assign rats to model (environmental stress-induced insomnia), model+DZP, or model+BLLZ groups. After five days of treatment, insomnia was induced by frequent cage changes (14:00, 16:00 and 18:00), and EEG changes were monitored. (4) Mechanistic study: 32 SD rats were randomly divided into the normal control group, model group, and model+DZP group. After five days of treatment, hypothalamic tissues were collected for biochemical analysis. γ-aminobutyric acid (GABA), glutamate (Glu), and dopamine (DA) levels were measured 
using biochemical kits while γ aminobutyric acid receptor subunit alpha-1 (GABAA1), core clock proteins period circadian regulator 2 (PER2) and circadian locomotor output cycles (CLOCK) protein expressions were assessed by Western blotting. RESULTS  (1) Compared with the normal control group, the sleep latency of BLLZ 10 and 20 g·kg^-1 and DZP groups was significantly shortened, and the locomotor activity of BLLZ 20 g·kg^-1 and DZP groups was significantly reduced; BLLZ 20 g·kg^-1 significantly increased the total sleep time, slow-wave sleep time, and average duration of sleep in normal rats, and significantly reduced the wakefulness time. (2) The total sleep time and slow-wave sleep time of the model group significantly decreased and the wakefulness time significantly increased compared with baseline. (3) Compared with the model group, the total sleep time and slow-wave sleep time of the model+BLLZ group and the model+DZP group were significantly increased, and the wakefulness time significantly shortened. (4) Compared with the normal control group, the Glu/GABA ratio, DA content and CLOCK protein expression were significantly increased and GABAA1 and PER2 protein expression were significantly decreased in the model group; compared with the model group, the Glu/GABA ratio, DA content and CLOCK protein expression were significantly decreased, and the expression of GABAA1 and PER2 were significantly increased in the model+BLLLZ group and the model+DZP group. CONCLUSION  BLLZ has sedative and hypnotic effects. It can prolong the total slow-wave sleep time by increasing the average duration of slow-wave sleep episodes, thereby increasing the total sleep time and improving environmental stress-induced insomnia. The mechanism may be related to the downregulation of the Glu/GABA ratio and DA levels as well as the enhancement of GABAA1 
expressions and the regulation of hypothalamic core clock protein expressions.

Sleep disorders are becoming a social problem that poses a danger to human health and an obstacle to economic development. As an endogenous hormone, melatonin plays an important role in regulating circadian rhythm. Research has shown that exogenous melatonin holds promising prospects in the treatment of insomnia disorders and thus merits in-depth exploration and development. Given the close relationship between pharmacokinetic properties and drug efficacy as well as toxicity, this paper reviews the quantitative analysis methods for melatonin and its major metabolites, especially the  pretreatment methods for biological samples and the related LC-MS/MS analysis methods. Studies related to the absorption, distribution, metabolism, excretion and drug-drug interactions of melatonin both in vivo and in vitro, as well as the level and species differences of endogenous melatonin are also outlined. Meanwhile, the druggability of melatonin and corresponding solutions are explored in the hope of providing data for the development of exogenous melatonin into novel sleep-regulating drugs.

OBJECTIVE To explore the ameliorative effect of ursolic acid on carbon tetrachlorideinduced acute liver injury in mice, and the feasibility of multispectral optoacoustic tomography (MSOT) for characteristic structural and functional imaging of liver tissues. METHODS Kunming mice were randomly divided into the normal control group, model group, model+ursolic acid 30, 60 mg·kg^-1 groups and model+bifendate 5.625 mg·kg^-1 group, with 14 mice in each. Each group was given the corresponding drug once daily for 7 days. An acute liver injury model was established in mice by intraperitoneal injection of 0.2% carbon tetrachloride in olive oil solution after the last administration. Blood was collected, liver tissues were taken 24 h after modeling, and the liver index was calculated, 8 mice from each group and the levels of serum glutamic pyruciv transaminase (GPT) and glutamic oxaloacetic transaminase (GOT), as well as superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in liver tissues were measured. The enzyme-linked immunosorbent assay (ELISA) method was used to detect the level of α - glutathione S-transferase (α -GST) in serum. The histopathological changes of the liver were observed under a light microscope. The remaining 6 mice in each group underwent MSOT technique was used for characteristic structural and functional imaging of liver tissue. Levels of oxygenated hemoglobin (HbO₂) and deoxygenated hemoglobin (Hb) were analyzed, oxygen saturation was calculated, and the extent of liver injury was assessed by examining the intrahepatic distribution of indocyanine green (ICG). RESULTS Compared with the normal group, the levels of GPT, GOT and α-GST in serum, content of MDA in liver tissues and the liver index in the model control group were significantly increased while the activity of SOD in liver tissues were significantly decreased. Compared with the model group, ursolic acid in each dose group significantly reduced the liver index of mice, lowered the serum levels of GPT and GOT as well as the level of α- GST, decreased the content of MDA in liver tissues, and elevated the activity of SOD in liver - injured mice. Hematoxylin-eosin staining showed that significant steatosis and hepatocyte necrosis and inflammatory cell infiltration in hepatocytes of mice in the model group. Ursolic acid significantly attenuated the degree of hepatocellular lesions and markedly reduced steatosis in mice. MSOT imaging showed that the HbO2 level and oxygen saturation were significantly lower while the Hb level was remarkably higher in the liver of mice in the model group. In each administration group, the level of HbO₂ significantly increased, the level of Hb was significantly decreased, oxygen saturation was significantly increased in the liver of model mice and the accumulation of ICG dye probe was atten⁃ uated in the body after hepatocyte injury. CONCLUSION Ursolic acid can elevate the hepatic oxygen saturation, improve the metabolism of ICG, reduce the degree of hepatic necrosis in mice, and help protect against carbon tetrachloride-induced hepatic injury in mice. The mechanism is probably related to the inhibition of oxidative stress.

The quality of toxicological data is critical to toxicity assessment and risk prediction. Integrating multiple streams of evidence and enhancing the quality of toxicological data are indispensable for more efficient and precise health risk assessment. There are several commonly used toxicology data quality evaluation systems abroad, which include Klimisch rating system, ToxRTool evaluation tool, the SciRAP evaluation tool and the integrated risk information system tool. The TRAM reliability evaluation tool is developed in China for toxicology data in food safety risk assessment.  These evaluation tools have different backgrounds and applicability of evaluation standards. Each evaluation system also has its advantages and disadvantages.

Peptide nanomedicines have been rapidly developed in the field of tumor treatment and the discovery of new drugs due to their strong stability, precise targeting and high bioavailability. The administration methods of peptide nanomedicines include intravenous injection, oral administration, respiratory administration and transdermal administration, among which intravenous administration is the most commonly used. Once inside the body, peptide nanomedicines can be transported everywhere via the blood circulation, accumulating in large quantities at the target site. Effective drug tracking analysis methods can help to obtain accurate pharmacokinetic data, while a comprehensive understanding of the in vivo distribution and pharmacokinetic characteristics of peptide nanomedicines can contribute to the development of new drugs and promote the progress of cancer therapy. This paper reviews the advantages and disadvantages of analytical methods for peptide nanomedicines and the research progress in their metabolism and transport in order to provide references for subsequent research on peptide nanomedicines.

Acral melanoma (AM) is a distinct and aggressive subtype of melanoma characterized by high metastatic potential and poor prognosis. The pathogenesis and therapeutic strategies for advanced AM differ significantly from those of other melanoma subtypes, yet AM has received relatively less attention. AM exhibits high heterogeneity and a low tumor mutation burden. Mutations in braf, nras, and the tert promoter occur at much lower frequencies in AM than in cutaneous melanoma, limiting the 
effectiveness of treatments such as recombinant B-Raf proto oncogene serine/threonine protein kinase(BRAF) inhibitors. Additionally, reduced tumor immunogenicity due to low tumor-infiltrating lymphocyte levels contributes to the limited efficacy of immune checkpoint inhibitors, including anti-programmed death-1 and anti-cytotoxic T-lymphocyte-associated protein 4 therapies. Recent discoveries of novel therapeutic targets, such as receptor tyrosine kinases and cyclin-dependent kinases, along with emerging immune checkpoints, including V-domain immunoglobulin suppressor of T cell activation, adenosine A2A receptor, T cell immunoglobulin and ITIM domain, and T cell immunoglobulin and mucin-domain containing-3, offer new prospects for improving AM patient outcomes. Many AM treatments remain in the experimental stage, with research focusing on small-molecule targeted therapies, immune checkpoint inhibitors, and tumor microenvironment modulation. Combination strategies incorporating next-generation cell therapies, oncolytic virus therapies, and therapeutic vaccines are also gaining prominence. Notably, clinical trials of personalized mRNA cancer vaccines have been promising, while antibody-drug conjugate and radionuclide-conjugated therapies present additional opportunities for enhancing AM prognosis. This article summarizes the cellular immune characteristics, mutation profiles, and tumor microenvironment of AM, as well as the current therapeutic strategies and advancements in the hope of expanding clinical benefits for AM patients.

OBJECTIVE  The inhibitory effect of active ingredients of Tripterygium wilfordii Hook. F. (TWHF) (celastrol, triptolide, triptonide, wilforlide A, wilforgine and wilforine) on human carboxylesterase 1 (CES1) and CES2 was detected to investigate the herb-drug interactions (HDIs) of TWHF. METHODS  Human liver microsomes catalysed hydrolysis of 2-(2-benzoyl-3-methoxyphenyl) benzothiazole (BMBT) and fluorescein diacetate (FD) were used as the probe reaction to phenotype the activity of CES1 and CES2, respectively. The residual activities of CES1 and CES2 were detected by ultra-high performance liquid chromatography (UPLC) after intervention with celastrol, triptolide, triptonide, wilforlide A, wilforgine and wilforine (100 μmol·L^-1). Kinetics analysis, involving half inhibitory concentration (IC₅₀), inhibition type and kinetic parameter (K_i), and in vitro-in vivo extrapolation (IVIVE), was carried out to predict the HDIs between these compounds and CES-metabolizing drugs. Molecular docking was performed to analyze the ligand-enzyme interaction. RESULTS  Out of the six main constituents of TWHF, only celastrol exhibited strong inhibition towards both CES1 and CES2, with the inhibitory rates of 97.45% (P<0.05) and 95.62% (P<0.05) , respectively. The IC₅₀ was 9.95 and 4.02 mol·L^-1, respectively, and the types of inhibition were all non-competitive inhibition. Based on the kinetics analysis, the K_i  values were calculated to be 5.10 and 10.55 μmol·L^-1 for the inhibition of celastrol on CES1 and CES2, respectively. IVIVE indicated that celastrol might disturb the metabolic hydrolysis of clinical drugs in vivo by inhibiting CES1. Molecular docking results showed that hydrogen bonds and hydrophobic contacts contributed to the interaction of celastrol and CESs. CONCLUSION  The inhibitory effect of celastrol on CES1 and CES2 might cause HDIs with clinical drugs hydrolysed by CESs.

Methamphetamine (METH) is a highly addictive synthetic psychoactive drug that is often abused as a psychostimulant. Chronic exposure to METH induces neurotoxic effects through oxidative stress, impairment of mitochondrial function, activation of astrocytes and microglia, and amino acid excitability. METH abuse increases the chance of developing neurodegenerative diseases such as Alzheimer′s disease, Parkinson′s disease, Huntington′s disease. However, the mechanism behind METH-induced neurotoxicity remains incompletely understood. So far, there is no specific treatment for METH-induced neurotoxicity. This paper reviews some of the potential mechanisms of METH-induced neurotoxicity in recent years, such as neuroinflammation, glutamatergic excitotoxicity, oxidative stress, and mitochondrial toxicity, and discusses the current therapeutic strategies related to mitigating the neurotoxic effects of METH in the brain through different pathways.

OBJECTIVE  To investigate the effect of N-methyl-D-aspartic acid (NMDA) receptor in the secondary visual cortex (V2) on methamphetamine-associated contextual learning and memory. METHODS  With male C57BL/6J mice as subjects and using the mouse conditioned place preference (CPP) experiment, the scores of CPP were observed after microinjection of NMDA receptor selective antagonist D-AP5 (0.5 μg per side) into the bilateral V2 during the formation phase, single microinjection of D-AP5 (0.5 μg per side) into the bilateral V2 prior to the expression test, and methamphetamine (0.5 mg∙kg^-1, ip)-induced reactivation test and methamphetamine-associated contextual-induced reactivation test, to evaluate the effect of NMDA receptors on the formation, expression and reinstatement of methamphetamine-induced CPP. RESULTS  Compared with the control group, microinjection of D-AP5 (0.5 μg per side) into the bilateral V2 during the formation phase did not have significant inhibitory effect on CPP scores, nor did single microinjection of D-AP5 (0.5 μg per side) into the bilateral V2 prior to the expression test, single microinjection of D-AP5 (0.5 μg per side) into the bilateral V2 prior to methamphetamine (0.5 mg∙kg-1, ip)-induced reactivation test or single microinjection of D-AP5 (0.5 μg per side) into the bilateral V2 prior to methamphetamine-associated contextual-induced reactivation test. CONCLUSION  The NMDA receptor in the V2 is not involved in the formation, expression and reactivation of methamphetamine-associated contextual learning and memory.

Commonly used anesthetic sedatives (opioids, benzodiazepines, ketamine, propofol, etc.) share the risk of inducing respiratory depression, and their multi-target mechanism of action presents significant heterogeneity. Opioids inhibit the rhythmic activity of the respiratory center of the medulla bulbar (such as the PreBötzinger complex and parbrachial nucleus) by activating both the μ-opioid receptor and the G-protein-gated inwardly-rectifying potassium channel and β-arrestin signaling pathway, resulting in decreased respiratory frequency and amplitude. Benzodiazepines enhance inhibitory neurotransmission mediated by γ-aminobutyric acid receptors, reduce the sensitivity of chemoreceptors to PaCO₂ and PaO₂, and lead to a decreased tidal volume and upper airway obstruction. Ketamine inhibits respiratory drive and respiratory muscle function by blocking N-methyl-D-aspartic acid  receptors and indirectly affecting the μ-opioid receptor. In addition, propofol inhibits pre-expiratory neuronal activity and relaxes upper airway muscles by activating the GABA_A receptor β₃ subunit. Currently, specific antagonists (naloxone/flumazenil) and respiratory stimulants (doxapram) are clinically used to treat respiratory depression, but they have defects such as short duration of action and insufficient specificity. The development of novel stimulants targeting μ-opioid receptor agonists and the D-serine release pathway of astrocytes, as well as broad-spectrum antidotes based on "molecular cage" technology, has become a new sphere of research that aims at precisely reversing respiratory depression while preserving analgesic and sedative effects. This article reviews the biological mechanisms of respiratory depression caused by sedative hypnotic anesthetic drugs, explores the advantages and disadvantages of treatments currently availabe, and proposes new strategies for improving respiratory depression in the future.

Over the past 30 years, nano-drug delivery systems (NDDS) have become a promising field of drug research. However, a poor knowledge of the in vivo process of NDDS, the limited methods of pharmacokinetic correlation, and the inability to effectively provide strong support for the construction of upstream drug as well as the evaluation of downstream pharmacology and toxicology have become the technical bottleneck for their clinical transformation. Lipid nanodrug (LND) is the most successful NDDS for industrial transformation with great biocompatibility. Taking LND as an example, this paper reviewed the delivery process and influencing factors in vivo, and summarized the regulatory mechanism of biological environments on drug release in vivo. Based on advanced spectroscopy and mass spectrometry techniques, the spatial and temporal distribution of the dynamic carrier particle/depolymerized molecule ratio and dynamic free/encapsulated drug ratio of LND in biological matrix were analyzed. Finally, the existing problems and future developments in this field were summarized to provide references for the analysis of NDDS in vivo, and stimulate readers' interest in nanomedical research and development. 

OBJECTIVE  To investigate the effects of ginsenosides as P-glycoprotein (P-gp) substrates in combination with paclitaxel on the proliferation and migration of colon cancer Caco-2 cells.  METHODS  Bio-layer interferometry (BLI) technology was used to detect the constants of ginsenosides and P-gp. Network molecular docking was adopted to predict the binding affinity energy of ginsenosides and P-gp. Caco-2 cells were divided into paclitaxel 0, 6.25, 12.5, 25, 50, 100 and 200 mg·L^-1 groups, ginsenoside Rg3 0, 6.25, 12.5, 25, 50, 100 and 200 mg·L^-1 groups, and paclitaxel 5 mg·L^-1+ginsenoside Rg3 0, 25, 50, 100 and 200 mg·L^-1 groups. After 48 h of incubation, the growth inhibition rate of Caco-2 cells was detected by MTT assay, and the interaction between the two drugs was quantitatively evaluated using the "one-belt, one-line" modle. Caco-2 cells were divided into the cell control group, paclitaxel 5 mg·L^-1 group, ginsenoside Rg3 50 and 100 mg·L^-1 groups, and paclitaxel 5 mg·L^-1+ginsenoside Rg3 50 and 100 mg·L^-1 groups. After 24 h of incubation, the proliferation and migration ability of the cells were detected by colony assay and Transwell migration assay. Caco-2 cells were then divided into the cell control group, quinidine 12.5 mg·L^-1 group, and ginsenoside Rg3 6.25 and 12.5 mg·L^-1 groups. After  4 h of incubation, the expression levels of P-gp and total protein were detected by ELISA. RESULTS  The affinity constants of ginsenoside Rb1, Rg3, Rg5 with P-gp were all less than 10^-3 mol·L^-1, while that of ginsenoside CK with P-gp was 10^-2 mol·L^-1. There was no typical binding dissociation curve between ginsenoside Re and P-gp. The absolute binding affinities of ginsenosides Rg3 and Rg5 to P-gp were determined to be 8.5 kcal·mol^-1 and 7.6 kcal·mol^-1, respectively. Ginsenosides mixed with PTX 5 mg·L^-1 inhibited the growth of colon cancer cells through synergy and addition, and the dose range of the synergistic effect was [0+5, 43.15+5] mg·L^-1; [164.51+5, 200+5] mg·L^-1, the additive effect dose ranged from [43.15+5, 164.51+5] mg·L^-1. The combination of the two drugs could significantly reduce the proliferation and migration ability of Caco-2 cells (P<0.01). The ELISA results showed a decrease in total protein and P-gp content in both the ginsenoside and quinidine groups (P<0.05). CONCLUSION  Ginsenoside bind to and inhibit the activity of P-gp, synergizing with paclitaxel to reduce the proliferative and migratory abilities of Caco-2 cells. The combination of ginsenosides and paclitaxel enhances the sensitivity of Caco-2 cells to paclitaxel induced inhibition. The combined use of these two substances is expected to achieve better anticancer effects compared to paclitaxel alone.

OBJECTIVE  To investigate whether aldo-keto reductases (AKRs) can act as a nitroreductase (NR) and bioactivate aristolochic acid Ⅰ (AA-Ⅰ) to produce AA-Ⅰ-DNA adducts. METHODS  ① Human-induced hepatocytes (hiHeps) and human bladder RT4 cells were used as tool cells and treated with AA-Ⅰ 0, 0.5, 1.0 and 2 μmol·L^-1 for 24 h. Cell viability was detected using the CCK-8 method, and the half maximal inhibition concentration (IC₅₀) was calculated using the CCK-8 method and the level of DNA adduct production was calculated. ② hiHeps and RT4 cells were treated with AKR inhibitor luteotin (0, 5, 10 and 25 μmol·L^-1)+AA-Ⅰ 0.2 and 1.0 μmol·L^-1 for 24 h, respectively, and the levels of DNA adducts were detected by a liquid chromatography-tandem mass spectrometer (LC-MS/MS). ③ hiHeps cells were incubated with 80 nmol·L^-1 small interfering RNAs (si-AKRs) for 48 h and treated with AA-Ⅰ1.0 μmol·L^-1 for 24 h. Real-time qualitative PCR (RT-qPCR) method was used to detect the mRNA expression of AKRs gene and LC-MS/MS technology was used to investigate the effect of specific AKR gene knockdown on DNA adduct levels. ④ 500 nmol·L^-1 human AKR recombinant proteins AKR1A1 and AA-Ⅰ were incubated in vitro under anaerobic conditions and the formation of AA-Ⅰ-DNA adducts was detected. RESULTS  ① The IC₅₀ of AA-Ⅰ to hiHeps and RT4 cells was 1.9 and 0.42 μmol·L^-1, respectively. The level of DNA adduct production of the two cell lines was significantly different (P<0.01). ② Luteolin≥5 μmol·L^-1 significantly inhibited the production of AA-Ⅰ-DNA adducts in both cells (P<0.05), and there was a concentration-dependent effect in hiHeps cells (P<0.01, R=0.84). ③ In the AKR family, the knockdown of AKR1A1 gene up to 80% inhibited the generation of AA-Ⅰ-DNA adducts by 30%-40%. ④ The AA-Ⅰ-DNA adducts were detected in the incubation of recombinant protein AKR1A1 and AA-Ⅰ under anaerobic conditions in vitro, approximately 1 adduct per 107 nucleotides. CONCLUSION  AKR1A1 is involved in AA-Ⅰ bioactivation, providing a reference for elucidation of the carcinogenic mechanism of AA-Ⅰ.

OBJECTIVE  To investigate the effect and underlying mechanism of hydroxytyrosol (HT) on mouse chondrocyte injury induced by oxidative stress. METHODS  Mouse chondrocytes were incubated with varying concentrations of HT 0-400 μmol·L^-1 for 24 h, and the viability of the mouse chondrocytes was assessed using CCK-8 kit. An oxidative stress model of chondrocytes was established by the addition of H₂O₂ 200 μmol·L^-1. The experimental groups included the cell control group, H₂O₂ group, and H₂O₂+HT 10, 50 and 250 μmol·L^-1 groups. After 24 h, the mRNA expression levels of interleukin-6 (IL-6), cyclooxygenase-2 (COX-2), prostaglandin E₂ (PGE₂), inducible nitric oxide synthase (iNOS), matrix metalloproteinase-3 (MMP-3 ), MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4 ), ADAMTS-5, SRY-box transcription factor-9 (SOX-9 ) and aggrecan (ACAN ) in mouse chondrocytes were detected by real-time quantitative PCR, the intracellular reactive oxygen species (ROS) level in chondrocytes was measured with 2, 7-dichlorodihydrofluorescein diacetate (DCFH-DA) staining, while the mitochondrial membrane potential was evaluated using JC-1 staining. After 48 h, the protein expression levels of iNOS, COX-2, MMP-13, and typeⅡcollagen (Col-2) in mouse chondrocytes were detected using Western blotting. RESULTS  HT at concentrations≤350 μmol·L^-1 had no significant effect on the survival of mouse chondrocytes. Compared with the cell control group, after 24 h, the mRNA expression levels of IL-6, COX-2, PGE2, iNOS, MMP-3, MMP-13, ADAMTS-4 and ADAMTS-5 in the chondrocytes of mice in the H₂O₂ group were increased, while the mRNA expression levels of SOX-9 and ACAN were decreased. Additionally, there was an elevation in the ROS level and a significant loss of mitochondrial membrane potential in the chondrocytes of mice. Compared with the H₂O₂ group, after treatment with HT 10, 50 and 250 μmol·L^-1, there were significant decreases in mRNA expression levels of IL-6, COX-2, PGE2, iNOS, MMP-3, MMP-13, ADAMTS-4 and ADAMTS-5, the mRNA expressions of SOX-9 and ACAN were increased, the ROS level was lowered. After treatment with HT 50 and 250 μmol·L^-1, the loss of mitochondrial membrane potential was ameliorated. Compared to the cell control group, the protein expressions of iNOS, COX-2 and MMP-13 were upregulated in the H₂O₂ group, while the protein expression of Col-2 was downregulated after 48 h. Compared to the H₂O₂ group, treatment with HT at concentrations of 10, 50 and 250 μmol·L^-1 resulted in decreased protein expressions of iNOS, COX-2 and MMP-13 in mouse chondrocytes, but the protein expression of Col-2 increased following treatment with HT 50 and 250 μmol·L^-1. CONCLUSION  HT can ameliorate H₂O₂-induced chondrocyte injury by reducing intracellular ROS levels and alleviating the loss of mitochondrial membrane potential, suppressing the release of inflammatory cytokines, inhibiting catabolic processes, and promoting anabolic activities.

New approach methodologies (NAMs) based next generation risk assessment for toxicity testing has quietly emerged globally. As an important component of NAMs, quantitative in vitro to in vivo extrapolation (QIVIVE) has come to be one of the significant technologies for chemical toxicity testing and risk assessment, and has gained rapid development and wide applications in the field of toxicology. The implementation of QIVIVE technology relies on two vital branches of computational toxicology: physiologically based toxicokinetics and physiologically based toxicodynamics. The system integrates population research, in vivo/in vitro testing, and computational toxicology research to quantitatively study the nonlinear quantitative relationship between exposure doses and physiological response, providing an efficient and accurate means of toxicity testing for health risk assessment of chemicals. At the same time, utilizing increasingly mature multi omics technologies to obtain high-throughput and 
effective toxicology information, and drawing a signal network of toxicity occurrence and development in the context of big data can greatly improve the efficiency and range of toxicology research.

OBJECTIVE  To investigate the protective effect of interleukin-35 (IL-35) mRNA-lipid nanoparticles (LNP) against lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice. METHODS  Fifity-six mice were randomly divided into 7 groups with 8 mice in each, including the normal control group, IL-35 mRNA-LNP (250 μg·kg^-1) group, LPS group, LPS+IL-35 mRNA-LNP (50,125 and 250 μg·kg^-1) group and LPS+Dexamethasone (DXM) group. Except for the normal control group and IL-35 mRNA-LNP (250 μg·kg^-1) group and ALI model was established by tracheal infusion of LPS in each of the other groups. IL-35 mRNA-LNP (250 μg·kg^-1) group and LPS+IL-35 mRNA-LNP (50,125 and 250 μg·kg^-1) group were injected with a corresponding dose of LNP encapsulated mRNA complex via the tail vein while the LPS+DXM group was injected with DXM via the tail vein. Lung coefficient and the wet to dry weight ratio (W/D) of lung tissue were recorded. The mRNA levels of inflammatory cytokines tumor necrosis factor-α (TNF-α), Interleukin-6 (IL-6  ) and IL-1β of lung homogenates were detected by real-time fluorescence quantitative PCR (RT-qPCR). LDH activity of lung homogenates and the protein levels of IL-35, TNF-α, IL-6 and IL-1β in lung homogenate were detected by corresponding kits. Hematoxylin-eosin (HE) staining was used to observe and analyze the pathological injury to lung tissue. The expression of Lymphocyte antigen 6G (Ly6G) was detected by Immunofluorescence to reflect the infiltration of neutrophils. RESULTS  Compared with the normal control group, LPS group and LPS+DXM group, IL-35 protein expression levels in lung homogenates of the other groups were more significant (P<0.01). Compared with the normal control group, lung coefficient, W/D ratio of lung tissue, LDH activity, mRNA levels and the protein levels of TNF-α, IL-6 and IL-1β in lung homogenates were significantly increased in the LPS group (P<0.01), accompanied by alveolar hemorrhage, alveolar wall thickening and neutrophils infiltration. After IL-35 mRNA-LNP administration, lung coefficient, W/D ratio of lung tissue, LDH activity, mRNA levels and the protein levels of TNF-α, IL-6 and IL-1β in lung homogenates were significantly decreased (P<0.01), and alveolar hemorrhage, alveolar wall thickening and neutrophil infiltration were obviously improved. CONCLUSION  IL-35 mRNA-LNP can express IL-35 protein in lung tissue of mice, and effectively improve LPS-induced ALI in mice by inhibiting the expression of proinflammatory factors.

OBJECTIVE  To investigate the protective effect of farrerol against lipopolysaccharide (LPS) induced acute lung injury (ALI) in mice and the mechanisms. METHODS  ① ICR rats were randomly  divided into the normal control group, model group and model+farrerol group, with 6 rats in each. The normal control group and model group were given an equal amount of normal saline intragaically for 4 d. The model+farrerol group was given 20 and 40 mg·kg^-1 intragaically for 4 d, while the normal control group was given an equal amount of normal saline intragaically on the 5^th day. The model group and model +farrerol group were injected with 3 mg·kg^-1 LPS solution for 24 h to construct an animal model of ALI. ② Mouse alveolar macrophages (MH-S) were cultured and randomly divided into the cell control group, model group and model+farrerol group.  Cell control group (conventional culture for 24 h), model group (100 μg·L^-1 LPS combined with 40 μg·L^-1 IFN-γ co-incubation for 24 h), model+farrerol group (5, 10 and 20 μmol·L^-1 farrerol pretreatment of MH-S cells for 24 h, and then 100 μg·L^-1 LPS combined with 40 μg·L^-1 IFN-γ co-incubation for 24 h), an inflammatory cell model was established in vitro. HE was used to observe the pathological changes of the lungs. The wet-dry weight ratio (W/D) was used to assess pulmonary edema while Evans blue dye (EBD) was used to detect pulmonary vascular permeability. The activity of myeloperoxidase (MPO) was detected by colorimetry. The number of white blood cells in BALF was counted with a blood cell counting plate. Cell proliferation assay (CCK-8) was used to determine the cell viability. The levels of interleukin-1β (IL-1β), tumor necrosis factor α (TNF-α), IL-6 and IL-10 in lung tissue, bronchoalveolar lavage fluid (BALF) and cell supernatants were detected via enzyme-linked immunosorbent assay (ELISA). The protein levels of inducable nitric oxide synthase (iNOS), arginase 1 (ARG1), phosphorylated NF-κB p65, NOD-like receptor protein 3 (NLRP3), cysteinyl aspartate specific proteinase1 (caspase1) and gasdermin family member (GSDMD-N terminal) in lung tissue and MH-S cells were detected with Western blot. RESULTS  ① Compared with the normal control group, the histopathological changes, Injury score, W/D ratio, pulmonary vascular permeability, MPO content, leukocyte count, pro-inflammatory factor IL-1β, TNF-α, IL-6 levels, iNOS, phosphorylated NF-κB p65, NLRP3, caspase1 and GSDMD-N-terminal protein expression in lung tissues were significantly increased in the model group (P<0.05, P<0.01) while the expression levels of anti-inflammatory factors IL-10 and ARG1 were decreased (P<0.05, P<0.01). Compared with the model group, the Injury score, W/D ratio, pulmonary vascular permeability, leukocyte number, MPO content, proinflammatory factor content and iNOS, phosphorylated NF-κB p65, NLRP3, caspase1 and GSDMD-N-terminal protein levels were significantly decreased in the model+farrerol group(P<0.05, P<0.01) while the levels of anti-inflammatory factor IL-10 and ARG1 protein were increased (P<0.05,P<0.01). ② The results of in vitro experiments showed that compared with the cell control group, the contents of IL-1β, TNF-α and IL-6 and the expression levels of iNOS, phosphorylated NF-κB p65, NLRP3, caspase1 and GSDMD-N-terminal protein were increased (P<0.05, P<0.01), and that the content of anti-inflammatory factor IL-10 and expression level of ARG1 protein were decreased (P<0.01). Compared with the model group, the content of proinflammatory factor and the expressions of iNOS, phosphorylated NF-κB p65, NLRP3, caspase1 and GSDMD-N protein in the model+farrerol group were significantly decreased (P<0.05, P<0.01) while the expression levels of IL-10 and ARG1 protein were increased (P<0.05, P<0.01). CONCLUSION  Farrerol can alleviate acute lung injury induced by LPS in mice, possibly by inhibiting the phosphorylation of NF-κB p65 and activation of NLRP3 inflammatome, alleviating pyroptosis of cells and regulating macrophage polarization.

OBJECTIVE  To study the way in which hypidone hydrochloride (code: YL-0919) improves motor function after ischemic stroke (IS) and explore the related mechanism. METHODS  Adult male SD rats were used to establish a middle cerebral artery occlusion (MCAO) model that simulated acute IS. All animals were randomly divided into four groups: sham group, MCAO group, MCAO+YL-0919 group, and MCAO+YL-0919+erastin (Era, ferroptosis inducer) group. The drug administration groups received the first ip injection 6 h after operation, followed by continuous ip injection once per day. After 7-10 d of drug administration, the effect of YL-0919 on motor function after IS were evaluated via neurological function test, adhesive-removal test, rotarod test, balance beam test and open field test. After 7 d of drug administration, TTC staining was used to detect the cerebral infarction area while the colorimetry method was used to measure the contents of glutathione (GSH), malondialdehyde (MDA), and ferrous ions (Fe²⁺) in the penumbra of the cerebral cortex. Western blotting was used to detect the expression levels of glutathione peroxidase 4 (GPX4), solute carrier family 7 member 11 (xCT), acyl-CoA synthetase long-chain family member 4 (ACSL4), and transferrin receptor 1 (TFR1) in the cortical penumbra. RESULTS  Compared with the sham group, the MCAO group showed higher neurological function scores (P<0.01), with notably prolonged time for tape removal and first contact with the right forepaw (P<0.01), spent significantly more time crossing the balance beam (P<0.01) but endured a notably shorter duration on the rotarod (P<0.01), reduced the movement distance in the open field (P<0.01), had a remarkably increased infarct area (P<0.01) but significantly level of GSH in the cortical penumbra region decreased (P<0.01), while MDA and Fe²⁺ levels were markedly increased (P<0.01). Protein expression levels of GPX4 and xCT were reduced (P<0.05), while those of ACSL4 and TFR1 were elevated (P<0.05). Compared with the MCAO group, these changes were significantly reversed after YL-0919 administration. However, when Era and YL-0919 were administered simultaneously, the reversal effect of YL-0919 was significantly weakened. CONCLUSION  YL-0919 can improve motor function impairment and reduce cerebral infarction areas in rats after IS, and the mechanism may be related to the inhibition of ferroptosis.

OBJECTIVE  To heterologously express and purify bioscavenger organophosphorus hydrolase (OPH), and evaluate its ability to resist ethyl paraoxon poisoning in vivo. METHODS  The Escherichia coli expression strain of OPH was constructed and purified by Ni-column affinity chromatography and gel filtration chromatography, and the purified product was identified by mass spectrometry. The enzyme activity and kinetic constants (K_m, V_max, k_cat and k_cat/K_m) were measured using ethyl paraoxon as the substrate. Twelve SD rats were randomly divided into the experimental group and control group. The experimental group was given 1 mg·kg^-1 of OPH solution by iv administration while the control group was given the same volume of normal saline. After administration, the two groups were immediately sc administration with 2×LD₅₀ ethyl paraoxon (0.86 mg·kg^-1). The state of the rats was observed and the poisoning symptoms were scored. The survival rats were given 2×LD₅₀ ethyl paraoxon every 24 h, and the survival curve and symptom score chart were drawn according to survival and poisoning symptoms of the rats to evaluate the anti-organophosphorus poisoning ability of OPH. RESULTS  The expression strain of OPH in E. coli was successfully constructed. After two-step purification, a single band of OPH was obtained by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, indicating that OPH had high purity,and the prepared protein sequence was consistent with the target protein. For OPH, K_m=7.5×10^-5 mol·L^-1, V_max=2.2×10^-7 mol·L^-1·s^-1, k_cat=158.4 s^-1, k_cat/K_m=2.1×10⁶ L·mol^-1·s^-1. The rats in the control group showed obvious poisoning symptoms after being given 2×LD₅₀ ethyl paraoxon, and all rats died with in 15 min. The rats in the experimental group did not show poisoning symptoms after the first exposure, and the poisoning symptoms gradually deepened after continuous exposure until all the rats died on the 4th day. CONCLUSION  OPH with high purity is successfully prepared in this study, and OPH could effectively resist ethyl paraoxon poisoning in rats.

Osteosarcoma is the most common primary malignant bone tumor in children and adolescents. The current treatment methods include immunotherapy, gene therapy, targeted therapy and chemotherapy, but the prognosis is poor and prone to recurrence and metastasis. There is increasing evidence that the Wnt signaling pathway is involved in the entire process of osteosarcoma occurrence, development, metastasis, and prognosis, making it an attractive target. Current research on small molecule compounds related to the Wnt signaling pathway is making progress and attracting more attention so that targeting the Wnt signaling pathway with small molecule compounds has become a promising therapeutic strategy for osteosarcoma. This review focused on the Wnt signaling pathway and introduced the relationship between the Wnt signaling pathway and osteosarcoma, targeting small molecule compounds upstream and downstream of Wnt signaling, analyzed the classification and mechanism of action of related small molecule compounds, and summarized research progress. Targeting the Wnt signaling pathway, clarifying the specific mechanism, and an intimate knowledge of the complexity of Wnt signaling will help improve the formulation of clinical treatment plans for osteosarcoma, providing references and ideas for the development of anti-tumor drugs related to Wnt regulation.

As the longest and most widely distributed pair of nerves in the brain, the vagus nerve is involved in the regulation of many systems and organs. Recent studies have found that the vagus nerve may be involved in the occurrence of a variety of neuropsychiatric diseases by regulating the release of neurotransmitters (such as norepinephrine, 5-hydroxytryptamine, gamma-aminobutyric acid and acetylcholine) and regulating the immune system and gut-brain axis. This article focuses on the regulatory mechanisms of the vagus nerve on neurotransmitters, immune system function, and the gut-brain axis, as well the therapeutic advances in vagus nerve stimulation for neurological and psychiatric diseases such as epilepsy, depression and anxiety disorders. 

OBJECTIVE  To investigate the effect of dopamine receptor 3 (D₃R) on fear memory induced by intense electric shocks and the possible neurobiological mechanism. METHODS  ① To prevent pain threshold differences from influencing the effect of intense electric shocks, wild-type (WT) and D₃R knockout mice (D₃R^-/-) were used in the Hargreaves test to evaluate their basal pain threshold, with the paw withdrawal latency (PWL) as the observation index. ② WT and D₃R^-/- mice were divided into control groups and model groups, respectively. On the training day (the first day, D 1), the model groups received inescapable electric shocks (1.5 mA, 10 s, 10 s interval, 15 cycle) while the control groups did not. Contextual fear tests were conducted on D2, D7, D10, D14, and D16 after training, with the percentage of freezing time (FT) as the observation index to evaluate fear memory acquisition induced by contextual cues. On D17, after the model groups showed no more fear responses to contextual cues, they were re-stimulated with low-intensity current (0.5 mA, 10 s, 10 s interval, 15 cycle) to evoke fear memory. The two control groups did not receive any shocks. Contextual fear tests were conducted on day 18, and the FT% of each group was observed to evaluate fear memory retrieval induced by contextual cues. ③ Another cohort of WT and D₃R^-/- mice was used to further investigate the underlying neural mechanism, with the same grouping and treatment as in ②. Real-time dynamic changes in calcium signals of dopamine (DA) neurons in the ventral tegmental area (VTA) of WT and D₃R^-/- mice were detected using fiber photometry during electric shocks. The fluorescence area under the curve (AUC) was used as the indicator to quantify the excitability of DA neurons. RESULTS  ① In the Hargreaves test, there was no significant difference in PWL between D₃R^-/- mice and WT mice, indicating the two genotype mice had no significant differences in the basal pain threshold. ② Compared with the WT control group, the percentage of FT of the WT model group significantly increased on D2, D7, D10, and D14 (P<0.05). Compared with the D₃R^-/- control group, the percentage of FT of the D₃R^-/- model group significantly increased only on D2 and D7 (P<0.01). Meanwhile, the percentage of the FT of D₃R^-/- model group was significantly lower than in the WT control group on D2, D7, D10, and D14 (P<0.05, P<0.01). During memory recall (D18), the percentage of FT of the WT model and D₃R^-/- model groups significantly increased compared to their respective control groups (P<0.01, P<0.05), while the percentage of FT of D₃R^-/- model mice was significantly lower than that of WT model mice (P<0.01). ③ In the fiber photometry test, during the shock period, the calcium signals of DA neurons in the VTA of WT model and D₃R^-/-model mice rapidly increased within the first 2 s, and then gradually decreased between 2 to 10 s. The AUC within the 2 to 10 s interval was significantly lower in D₃R^-/- model mice compared to WT model mice (P<0.05), indicating that the excitability of DA neurons in the VTA of D₃R^-/-model mice was significantly lower than that of WT-model mice. CONCLUSION  D3R knockout inhibits the acquisition and recall of long-term fear memory in mice, and its neurobiological mechanism may be related to the decreased excitability of DA neurons during electric shock.