(]Ginsenoside Rg1 inhibits vascular intimal hyperplasia in balloon-injured ratcarotid artery by down-regulation of extracellular signal-regulated kinase 2
GAO Yang, DENG Jiang, YANG Dan-Li, GONG Qi-hai, HUANG Xie-Nan
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Department of Pharmacology and the Key Laboratory of Basic Pharmacology of Guizhou Province, Zunyi MedicalCollege, Zunyi 563000, China
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History+
Received
Revised
Published
1900-01-01
1900-01-01
2011-09-23
Issue Date
2020-07-22
Abstract
Ginsenoside Rg1(Rg1), one of the main active components of Panax ginseng, has been demonstrated to inhibit proliferation of vascular smooth muscle cells (VSMCs) induced by tumor necrosis factorα in vitro. The present study is aimed to examine the possible effects of Rg1 on vascular neointimal hyperplasia in ballooninjured carotid artery of rats in vivo. The animal model was established by rubbing the endothelia with a balloon catheter in the common carotid artery (CCA) of male Sprague Dauley rats. Then the rats were intraperitoneally injected with distilled water in model group and sham operation control, or with Rg1 4 mg·kg-1·d-1, 8 mg·kg-1·d-1 and 16 mg·kg-1·d-1in other balloon-injured groups. After consecutive 14 d, the vascular intimal hyperplasia was evidenced by histopathological changes of the CCA and the protein expression of proliferating cell nuclear antigen (PCNA) , a marker of the proliferation in VSMCs, and the phosphorylated extracellular signalregulated kinase2 (pERK2) as well as mitogenactivated protein kinase phosphatase1 (MKP-1) were examined by immunohistochemistry; the expressions of protooncogene (c-fos) , ERK2 and SM α-actin mRNA were analyzed by RealTime RT-PCR. The results demonstrated that Rg1 administration could significantly ameliorate the histopathology of CCA and decrease the protein expression of PCNA induced by endothelia rubbing; and Rg1 medication also significantly decreased the expressions of p-ERK2 protein, ERK2 and c-fos mRNA in vessel wall, but upregulated the MKP-1 expression, which was reported to inactivate mitogenactivated protein kinase pathway. Furthermore, Rg1 could elevate the decreased SM α-actin mRNA expression induced by balloon injury. It is concluded that Rg1 can suppress the vascular neointimal hyperplasia induced by balloon injury, the mechanism may be involved in the inhibition on ERK2 signaling, and related, at least partly, to the increase in MKP-1 expression.
